Texas AgriLife wheat breeders are researching how to access key traits in the wheat genome.
“Our goal is to develop improved wheat varieties with high yield capability and resilience to a variety of stressors across differing climates and water resource availability,” Dr. Shuyu Liu, AgriLife Research small grains geneticist in Amarillo said.
The study, recently published in the Crop Science Journal, included 3 wheat populations derived from 2 popular AgriLife Research cultivars, TAM 111 and TAM 112, and other diverse wheats.
The AgriLife Research study included teams led by Jackie Rudd and Amir Ibrahim, wheat breeders in Amarillo and College Station, respectively; Dirk Hays, plant geneticist in College Station; and Qingwu Xue, crop stress physiologist in Amarillo.
“We have talked about using genetic markers for many years now, but this research moves us from ‘proof of concept’ to actual practice in our TAM wheat germplasm,” Rudd said. “To be able to accelerate the movement of greenbug and wheat curl mite resistance from TAM 112 into new varieties is huge.”
Single nucleotide polymorphisms, known as SNPs, are the most common type of genetic variation in a plant, animal or human. Each SNP represents a difference in a single DNA building block, called a nucleotide.
Those variations are found throughout the wheat DNA and act as biological markers, helping scientists locate genes that are associated with a certain trait or characteristic.
The international wheat community, including the U.S., has developed an array chip with 90,000 SNP markers, Liu said. Markers are used to tag the trait so it is easily identifiable with new lines of wheat containing the key genes.
The 90,000 SNP array chip has become a common genetic marker method used by wheat breeders and geneticists, but there are limitations. Of the 90,000 markers, only about 40,000 of them had been mapped onto chromosomes, he said.
Mapping SNPs in wheat can help to develop high throughput molecular markers for important traits, Liu said.
Silvano Ocheya, a doctoral student of Liu’s who is completing his thesis research, has already mapped 6,000 of the SNPs, including those pinpointing drought tolerance and wheat streak mosaic virus characteristics, in one of the three mapping populations.
Liu’s lab has also developed high throughput molecular markers for greenbug and wheat curl mite resistance.
The AgriLife Research study confirmed 13,000 previously mapped SNPs in their three wheat population crosses, and they newly mapped 2,190 unique SNPs.
Liu said the 15,000 SNPs they have confirmed are being used to study complex agronomic traits such as yield, yield components and heat tolerance, in addition to disease and insect resistance controlled by single dominant genes.
They are also studying the interaction between the genes and the environments.
“For instance, we are working to identify major genes controlling yield under dryland and irrigation conditions,” Liu said. “Data is collected from multiple environments so we can focus on how major genes contribute to yield improvement under different climates.”
All this information will help breeders incorporate key genes associated with the traits and get the new enhanced varieties to producers sooner, he said.
The title of the published paper is “Validation of Chromosomal Locations of 90K Array Single Nucleotide Polymorphisms in US Wheat.”